LOIUS PASTEUR AND HIS ACHIEVEMENTS

MNEMONIC

                                        Father VARGAS

 

Father : Father of microbiology

V          : Vaccine term

A          : Abiogenesis theory was disproved by him

R          : Rabies vaccine

G          : Growth of bacteria on complex media

A          : Anthrax vaccine

S           : Sterilization technique 

DRACUNCULUS MEDINENSIS

Dracunculus medinensis...

                          Also known as Guinea worm is a nematode that causes dracunculiasis, also known as guinea worm disease.

                          - It is caused by the female nematode which usually measures about 30-31 inch in length andis one of the longest nematode infecting humans

                             - Adult worms cause skin inflammation and ulceration.

       Transmission :-

                              The disease is transmitted by drinking water containing the intermediate host

copepods in which the larvae live.
                       

      Diagnosis :- 

                              Dracunculiasis is diagnosed by finding the head of the worm in a skin lesion or
larvae that are released from a lesion following contact with water.

    Treatment :- 

                              The disease is treated by removing subcutaneous worms (formerly by winding them on a thin stick, now usually by surgery).

SELENITE F BROTH

SELENITE F BROTH....

                    It is recommended as enrichment media for the isolation of Salmonellae from feces, urine or other pathological materials.

             Appearance :

                                    Part A : White to light yellow homogeneous free flowing powder
                                    Part B : White to cream crystalline powder

             Colour and Clarity of prepared medium

                                    Cream to yellow colored clear solution without any precipitate

             Organisms showing cultural response 

                                    Escherichia coli
                                    Salmonella Typhi
                                    Salmonella Typhimurium
                                    Salmonella Choleraesuis


FOR DETAILED EXPLANATION ON SELENITE F BROTH, CLICK HERE 
     

CASTANEDA BIPHASIC MEDIUM

CASTANEDA BIPHASIC MEDIA...

                         Biphasic media: A culture system comprising of both liquid and solid medium i the same bottle.

                            This is known as biphasic medium (Castaneda system for blood culture).
 The inoculum is added to the liquid medium and when subcultures are to be made, the bottle is
simply tilted to allow the liquid to flow over the solid medium. This obviates the need for
frequent opening of the culture bottle to subculture.